| The identification of molecules present in plants by chromatographic techniques such as HPLC, can bring promising alternative to the control of plant diseases, since brassicas have glucosinolates, which act in plant defense. The objective of this work was to verify the presence of allyl isothiocyanate by HPLC (High Performance Liquid Chromatography Reverse Phase), using different methods of extraction. The plant material was leaves of Crambe abyssinica, dried at 45 °C for 48 h. The extraction methods used consisted of 200 mg L-1 of plant material for each method: infusion, aqueous solution, and cetonic, hydroalcoholic, methanol, hexane and chloroform solutions. The identification and quantification were by HPLC on C18 column UV at 244 nm. The experiment... |
